Mapping the male mouse chromatin accessibility landscape by UUATAC-seq

The current understanding of regulatory sequences in the vertebrate genome is still incomplete. By introducing an adapter switching strategy, we developed an ultra-throughput ultra-sensitivity single-nucleus ATAC-seq (UUATAC-seq) protocol that enables construction of high-quality chromatin accessibility landscape from one species in a one-day experiment. By using UUATAC-seq, we mapped open chromatin regions for five representative vertebrate species at the landscape scale. UUATAC-seq was performed across 23 tissues from one male mouse to profile over 290,000 nuclei. Mouse tissues were obtained from the C57BL/6J strain (8 weeks).