single cell RNA-sequencing on triple negative G422-glioma(G422TN)

The aim was to reveal cellular heterogeneity of orthotropic G422TN-glioma Overall design: Control group:The seventh day after G422TN(5*104) inoculation into the brain, brain tumor tissue was taken for single cell isolation. LTS group: After combined therapy, the mice survived for more than 100 days, and the contralateral side of the brain received the same tumor inoculation again. The tumor-bearing mice survived again for more than 100 days. At this time, on the 7th day after tumor inoculation, the tumor tissue was obtained for single cell sequencing.