Transcriptional Sequencing of Salmonella enterica serovar Typhimurium wild typle and DsrA-deletion mutant Survival unfer LB or H2O2 conditions

To elucidated the regulatory effect of DsrA on the oxidative resistance in Salmonella enterica serovar Typhimurium. RNA sample of wild-type and DsrA-deletion strain that grown in LB medium or H2O2 stress were extracted. Three biological replicates were carried out per sample. RNA sample was sequenced with Illumina HiSeq 4000 System. DESeq2 method was used to calculate the differentially expressed genes. qRT–PCR and gene mutation were used to validate RNA-seq result. wild-type in LB (WT_CK), wild-type in H2O2(WT_TREAT), dsrA mutant in LB(MT_CK), dsrA in H2O2 (MT_TREAT) Three biological repetitions of each samle, totally 12 samples were conducted RNA sequncing.