Medicago truncatula shoot metabolome analysis using stable isotopes. Medicago truncatula

Metabolites in the Medicago truncatula shoot are investigated by using stable isotope labelings. This analysis was performed to estimate the elemental composition with high reliability using the number of stable isotopes incorporated in the compound as an index and to add information to discuss the biosynthetic pathway. The Medicago seedlings were labeled with 13C, 15N, 18O, and 34S, respectively in the same condition.