Krüppel-like factor 5 regulates stemness, lineage specification, and regeneration of intestinal epithelial stem cells [RNA-seq]

The essential functions of intestinal stem cells (ISCs) are to self-renew and give rise to progenitors that subsequently differentiate to absorptive or secretory cells, thus maintaining homeostasis in the intestinal epithelium. In this study, we analyzed the transcriptomic and epigenetic changes of ISCs with Klf5 deletion to understand the role of KLF5 in ISC identity and functions. Overall design: RNA-sequencing and ChIP-seq analysis of control mouse intestinal stem cells and Klf5-deleted intestinal stem cells.