A shotgun proteomics reference map for the alkali-sensitive covalently-linked proteins of the cell wall of A. fumigatus during specific stages of early fungal development (dormant conidia, isotropically expanding conidia, early germ tube emergence and young hyphae).

Four different developmental time points representing the Afu development from dormant conidia into young hyphae (0h, 4h, 6h, 8h) were chosen. Cell walls of the Afu samples were extracted using denaturing methods followed by isolation of solubilized proteins. Samples were processed using filter aided sample preparation (FASP) / trypsin digestion. The peptides were analyzed by one-dimensional LC (capillary C18 nano-Eflow mode) on-line with a nano-ESI source and tandem MS (LTQ ion trap).