Dysregulated ac4C modification of mRNA in a mouse model of early stage Alzheimer's disease.

In this study, ac4C modifications in mRNA were investigated using six-month-old 5×FAD transgenic mice, a widely recognized model of Alzheimer's disease, along with age- and sex-matched wild-type (WT) controls. To elucidate the role of ac4C-modified mRNA in AD, we employed three analytical techniques: acetylated RNA immunoprecipitation sequencing (ac4C-RIP-seq), RNA sequencing (RNA-seq), and proteomic analysis. The first two were used to identify mRNAs carrying ac4C modifications and to quantify mRNA abundance, respectively. acetylated RNA immunoprecipitation -sequancing (acRIP-seq) was used to determine the acetylation modification of mRNA in the hippocampus from 6 month mice (3 5xFAD mice and 3 WT mice). Then IgG- sequancing was used to correct the result of acRIP-seq.