GNPS - Lemonade Creek soil heterotrophy experiment

Lemonade Creek soil samples. Algae colonize the soil just below the surface. Samples were taken in quadruplicate at four time points starting at 7:30 am (~30 min prior to sunrise) and finished at 8:00 pm (dark). Each replicate sample has both a pellet and supernatant. Soil material was scraped from the green layer beneath the surface, suspended in 10 mls sterile ultrapure water and shaken vigorously to suspend all biomass. Soil mineral material was allowed to settle (~10-15 sec), the supernatant was decanted to a fresh 15 ml Falcon tube, and then centrifuged (2100 x g, 5 minutes). Supernatant was poured off to a fresh tube and then it and the pellet (soil biomass plus some silt materials) were flash frozen in ethanol – dry ice bath and then stored on dry ice until transferred to -80°C freezer.

The work (proposal:https://doi.org/10.46936/10.25585/60000481) conducted by the U.S. Department of Energy Joint Genome Institute (https://ror.org/04xm1d337), a DOE Office of Science User Facility, is supported by the Office of Science of the U.S. Department of Energy operated under Contract No. DE-AC02-05CH11231.