Investigation on Ruthenium(II) Bipyridine/AgIII Complexes Chemiluminescence System and Its Application for Sensitive Norfloxacin and Ofloxacin Detection

The reaction mechanism of AgIII complex ([Ag(HIO6)2]5–) with ruthenium(II) bipyridine (Ru(bpy)32+) in acid medium was investigated. A novel flow injection chemiluminescence (CL) analysis method was developed for the detection of norfloxacin (NFLX) and ofloxacin (OFLX) in commercial drug, milk and human urine samples based on AgIII-ruthenium(II) bipyridine in acidic solution. Under optimal conditions, CL intensities were proportional to drug concentrations in the range of 1.6 × 10-9 to 8 × 10-6 g mL-1 for NFLX and 1.0 × 10-9 to 4 × 10-6 g mL-1 for OFLX. The detection limit was 1.0 × 10-9 g mL-1 for NFLX and 0.8 × 10-9 g mL-1 for OFLX. On the basis of the CL, UV and fluorescence spectra, possible reaction mechanisms were proposed.本研究对酸性介质中三价银配合物([Ag(HIO6)2]5–)与联吡啶钌(II)（Ru(bpy)32+）的反应机制进行了探究。基于酸性溶液中的三价银-联吡啶钌(II)体系，本研究开发了一种新型流动注射化学发光（Chemiluminescence, CL）分析法，用于检测市售药品、牛奶及人体尿液样本中的诺氟沙星（Norfloxacin, NFLX）与氧氟沙星（Ofloxacin, OFLX）。在最优实验条件下，化学发光强度与药物浓度呈良好线性相关：诺氟沙星的线性范围为1.6×10^-9 至8×10^-6 g·mL^-1，氧氟沙星为1.0×10^-9 至4×10^-6 g·mL^-1；二者的检测限分别为1.0×10^-9 g·mL^-1与0.8×10^-9 g·mL^-1。最后，结合化学发光、紫外及荧光光谱数据，本研究提出了该体系可能的反应机理。