Paediatric Saliva Protein Library (Burn patients and Healthy Controls) and SWATH analysis

A paediatric saliva protein spectral library was created using drool samples collected from children with small area burn injury as well as healthy controls. Iso-electric focussing, LDS-PAGE and a combination of both fractionation methods were utilised to increase the protein coverage of the library. This resulted in the identification of 1310 proteins at a 10% FDR cut-off. The library samples were analysed using DDA-MS. The generated protein spectral library was used to quantify the abundance of 742 proteins in each individual participant sample using SWATH-MS. All samples were run on a Sciex TripleTOF 6600 mass spectrometer, and the data was processed using the OneOmics Suite v3.0 (SCIEX). Index retention time proteins were used in this experiment. The effect of burn injury and various burn characteristics on salivary protein abundance profiles were evaluated.