Investigation of pneumococcal two-component signal transduction system RR/HK06 in TIGR4

We wanted to identify genes regulated by the two-component signal transduction system RR/HK06 in Streptococcus pneumoniae. We used microarray in order to discover regulated genes, investigating the effects of the over-expression of RR06 in TIGR4 (serotype 4). Keywords: Gene Regulation The RNA used for microarray analysis was extracted from S. pneumoniae TIGR4 over-expressing RR06 and TIGR4 containing over-expression vector without insert. RNA used was from three biological replicates with dye-swap performed for each.