HOXB13 lysine 13 acetylation regulated transcriptional targets in castration-resistant prostate cancer

To understand the mechanistic role of HOXB13 lysine acetylation in CRPC development andd progression, differential gene expression analysis was performed following RNA-sequencing of the HOXB13K13A mutant versus the isogenic parental C4-2B to identify bonafide transcriptional targets of acetylated HOXB13. Chromatin remodeling and self-renewal genes were significantly impacted as a result of HOXB13-K13 mutation. These results suggested that single-site acetylation in HOXB13 is critical and may even be sufficient to promote cellular reprogramming associated with PC pathogenesis. Total RNA was isolated from C4-2B parental and HOXB13K13A mutant clones and subjected to RNA -sequencing.