Datasets and metadata supporting the published article: Retinoid X receptor agonist LG100268 modulates the immune microenvironment in preclinical breast cancer models

The study investigated the effects of LG268, a Retinoid X Receptor (RXR) agonist, as an immune modulator in the tumor microenvironment of two genetically and histologically distinct mouse models of breast cancer.<br><b>Data access:</b> The datasets supporting all the figures in this published article are publicly available in the figshare repository (as part of this data record, <b>https://doi.org/10.6084/m9.figshare.9944942</b>) and in the supplementary files of the article.<br><br><b>Ethical approval:</b> All animal studies were done in accordance with protocols approved by the Institutional Animal Care and Use Committee at Michigan State University. <br><b>Study aims and methodology: </b>The present study aimed to examine the immunomodulatory effects of LG268, an RXR agonist, in MMTV-Neu and PyMT mouse models of human epidermal growth factor receptor 2 (HER2) positive breast cancer and triple negative breast cancer (TNBC), respectively.The following cell lines were cultured: RAW 264.7 mouse macrophage-like (ATCC) cells, E18-14C-27 cells, THP-1 (ATCC) cells, SK-BR-3 (ATCC) cells and THP-1 cells.Western blotting was performed on whole cell lysates of cells treated with drugs. Western blotting was also performed on homogenised tumors or mammary glands of mice treated with drug or a control diet.Flow cytometry was carried out on cells derived from the tumor, mammary gland and spleen removed from MMTV-neu or MMTV-PyMT female mice. For more details on the methodology, please see the related article. <br><b>Dataset descriptions:</b> The datasets supporting the figures in the published article are described in table <b>Leal et. al.xlsx </b>and are part of this data record. All four datasets are in .xlsx file format.<br><b>Figure 7 dataset.xlsx: </b>Data on the expression levels of PD-L1 in cancer cells and macrophages after treatment with LG268 or bexarotene.<br><b>MMTV-Neu immune and protein data.xlsx:</b> Data on MMTV-Neu mice including tumor and spleen weight, tumor immune cell populations, spleen immune cell populations and mammary gland immune cell populations.<br><b>PCR and flow CD3 activation.xlsx</b>: Quantitative PCR data to determine the levels of FOXP3 and data on CD3 T cells stimulated with anti-CD3 and treated with LG268. Activation of CD4 and CD8 was evaluated by flow cytometry<br><b>PyMT data sets.xlsx:</b> Data on PyMT mice, including tumor and spleen weight, tumor immune cells, mammary gland immune cells and spleen cells. <br>