Neutralizing potency of the TA12 mAb and its F(ab')<sub>2</sub> and PEG- F(ab')<sub>2</sub> fragments.
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The mAb neutralizing activity was determined using the mouse protection assay with 5 estimated MLD50/mouse of BoNT/A1. 5 estimated MLD50/mouse were incubated for 30 min at room temperature with 2,500 to 0.025 ng of mAb and the mixture (0.5 ml) was injected intraperitoneally in each mouse of a group of 8 mice. Results are expressed as the number of dead mice versus the total number of challenged mice and are from three independent experiments
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2010-08-26
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