DIET_MICROBIOTA_BLASTOCYSTIS

Supplementary materials: - Microbiota data: Dataset containing the relative abundances of bacterial genera identified through 16S rRNA sequencing from faecal samples. This file includes samples from the FACSA cohort (university students) and the PAVILA cohort (institutionalised children and caregivers), all colonised by Blastocystis spp. Metadata file: Comprehensive metadata for each sample, including cohort, age, sex, BMI, and HEI-2020 individual score. - Table S1. This table compares baseline characteristics and diet quality between the FACSA and PAVILA cohorts. - Table S2. Detailed comparison of Healthy Eating Index-2020 (HEI-2020) component scores between the two cohorts, highlighting differences in food group consumption and overall diet quality. - Top 10 taxa by principal coordinates analysis (PCoA): A list of the ten most representative bacterial genera associated with the principal coordinate axes that differentiate the FACSA and PAVILA cohorts. This analysis was based on Bray–Curtis dissimilarity matrices of Hellinger-transformed genus-level data. Taxa were identified using envfit projections with p < 0.1. These supplementary files provide additional detail and transparency for the analyses performed, particularly regarding the dietary and microbiota differences between the two socio-demographically distinct populations.补充材料： - 微生物组数据： 包含通过16S核糖体RNA（16S rRNA）测序从粪便样本中鉴定得到的细菌属相对丰度的数据集。本文件涵盖FACSA队列（大学生群体）与PAVILA队列（机构照护儿童及照护者）的所有样本，上述样本均定植有芽囊原虫（Blastocystis spp.）。 元数据文件： 包含所有样本的完整元数据，涵盖队列归属、年龄、性别、身体质量指数（Body Mass Index, BMI）以及健康饮食指数-2020（Healthy Eating Index-2020, HEI-2020）个体评分。 - 表S1： 对比FACSA队列与PAVILA队列的基线特征及饮食质量水平。 - 表S2： 详细对比两个队列的健康饮食指数-2020（HEI-2020）分项评分，着重阐明食物组摄入模式与整体饮食质量的差异。 - 基于主坐标分析（Principal Coordinates Analysis, PCoA）的前10类分类单元： 列出与区分FACSA队列及PAVILA队列的主坐标轴相关的10个最具代表性的细菌属。本分析基于经Hellinger转换的属水平微生物组数据构建的Bray-Curtis相异度矩阵，通过envfit投影法鉴定分类单元，显著性阈值设定为p < 0.1。 本批补充文件为本次研究的分析工作提供了更为详实的细节与透明度，尤其针对两个社会人口学特征迥异的群体之间的饮食与微生物组差异进行了补充说明。