Ethological profiling defines pain behaviors and parses analgesia from drug side effects in a mouse model of complex regional pain syndrome

Complex regional pain syndrome (CRPS) is a form of chronic post-injury pain affecting the extremities with contributions from the somatic and sympathetic nervous systems. The mouse tibial fracture-cast model was developed to enable preclinical study of CRPS mechanisms and guide condition-specific drug development. Given the inherent limitations of reflex pain measures in mice, we sought to holistically characterize pain-like behaviors in this model in neutral and aversive environments using quantitative behavior analysis with LabGym, a user-friendly automation tool that requires no special equipment or extensive computational resources. Here, we show that tibial fracture-cast injury causes distinct changes in naturalistic and nocifensive behaviors in male and female mice in neutral and aversive environments, demonstrating reliable behavioral categorization using robust learning-based holistic assessment. As proof-of-concept for therapeutic testing, we leveraged this behavioral evaluatio..., , # Data from: Ethological profiling defines pain behaviors and parses analgesia from drug side effects in a mouse model of complex regional pain syndrome Dataset DOI: [10.5061/dryad.dncjsxmd3](https://doi.org/10.5061/dryad.dncjsxmd3) ## Description of the data and file structure These files contain raw data generated and analyzed for the paper entitled, \"Ethological profiling defines pain behaviors and parses analgesia from drug side effects in a mouse model of complex regional pain syndrome.\" ### Files and variables #### File: Muwanga_Sourcedata_FIG2-8.xlsx **Description:** This Excel file contains raw data for the main figures 2-8. ##### Variables * Tab 1: Figure 2 * 2b) Mechanical sensitivity before and after tibial fracture-cast injury in male mice * 2c) Thermal sensitivity before and after tibial fracture-cast injury in male mice * 2d) Weight bearing before and after tibial fracture-cast injury in male mice * 2e) Paw width before and after tibial fracture-cast injur..., ,