RNA-Seq of hippocampus in founder mice

To understand the genetic regulation of gene expression and patterns of gene co-expression, we sequenced the transcriptome of the hippocampus of founder mice of both sexes. Mice were sacrificed between 6-8 weeks of age and hippocampus dissected. Total hippocampal RNA was isolated using a TRIzol Plus RNA purification kit (Life Technologies) and mRNA sequencing library was prepared using a TruSeq kit (Illumina), both according to manufacturer's protocols. Paired-end 100bp reads were obtained using the Illumina HiSeq 2000. transcription profiling by high-throughput sequencing design为解析基因表达的遗传调控机制与基因共表达模式，我们对不同性别的创始小鼠（founder mice）的海马体转录组进行了测序。实验小鼠于6至8周龄时处死，并对其海马体进行解剖分离。总海马体RNA采用TRIzol Plus RNA纯化试剂盒（Life Technologies公司）提取，mRNA测序文库则通过TruSeq试剂盒（Illumina公司）构建，所有操作均严格遵循厂商提供的实验规程。采用Illumina HiSeq 2000测序平台获取100bp双端测序读段。本研究采用高通量测序开展转录组分析的实验设计方案。