CDFF-model microcosm biofilm samples from human saliva Metagenome. human oral metagenome

The aim of this study was to assess the effect of the age, thickness, and cariogenicity on the amount of red fluorescence produced by in-vitro microcosm biofilms in a constant depth film fermentor (CDFF). The model was inoculated with saliva from one healthy donor. The composition of the biofilms was assessed using 454-pyrosequecing of the 16SrRNA gene. A shift in the microbial composition was seen in time as well as between the respective samples of the two sucrose frequencies.