Genome-wide binding of Bmal1 and Clock in adult mouse skeletal muscle.

The goal of this study was to define the binding sites of the core circadian factors, BMAL1 and CLOCK across the genome in adult mouse skeletal muscle. We found that mitochondrial inner-membrane genes were among the most enriched rhythmic genes via gene ontology analysis, and ChIP-Seq analysis corroborated the role of Clock and Bmal1 in targeting promoters of mitochondrial inner-membrane genes. We collected mouse gastrocnemius muscles from 10 adult C57BL/6J male at 2hrs after lights on (ZT2). Nuclei were extracted and proteins fixed and we used antibodies to Bmal1 and Clock for Chromatin IP. There are two biological replicates for Bmal1, two biological replicates for Clock, one input sample for Bmal1, and one input sample for Clock. Libraries were prepared using NEBNext Ultra DNA library kit and the libraries were sequenced on HiSeq3000 Illumina system. Each processed data was generated from both replicates and is linked to the corresponding rep1 sample records.