RNA sequencing of paVICs exposed to LPC and dantrolene

To investigate the gene expression changes due to LPC-induced calcific nodule formation in porcine aortic valve interstitial cells (paVICs). We then examined differential gene expression due to treatment with dantrolene, which inhibits calcific nodule formation, with and without LPC after 24 hours of exposure. Overall design: Comparative gene expression profiling analyis of RNA-seq data for primary paVICs.