Genome-wide chromatin immunoprecipitation sequencing (ChIP-seq) in a monoclonal population of HEK-293 cells stably expressing a Tet-OFF regulatory system (i.e. HEK293-PFL-TFEB).

In order to identify the direct target of TFEB, we performed a ChIP-seq in HEK293-PFL-TFEB cells where, after removal of tetracycline, samples were collected in duplicates at 18, 36 and 90 hours a ChIP-seq in HEK293-PFL-TFEB cells was performed after removal of tetracycline, samples were collected in duplicates at 18, 36 and 90 hours. Two ChIP samples for each timepoint and one immunoglobulin G (IgG) control samples were subjected to Illumina high-throughput sequencing and mapped to the reference human genome hg19