Single-cell analysis of the epigenomic and transcriptional landscape of innate immunity to seasonal and adjuvanted pandemic influenza vaccination in humans (TIV_scRNA-seq)

Human subjects were vaccinated with seasonal influenza vaccine (TIV, 2014-2015). We then used scRNA-seq to constructed the single-cell landscape of the innate immune response to TIV at the transcriptional level. PBMCs from vaccinated individuals were isolated at day 0, 1, and 30, then enriched for DC subsets using flow cytometry and analyzed using droplet-based single-cell gene expression profiling. Overall design: A total of 9 samples from 3 healthy individuals at 3 time points was analyzed: day 0 before vaccination, and day 1 and day 30 after vaccination. Individual abx-02 lacks d30 sample.