Isolation of lysosomal fractions from ES2 cancer cells

Pro-metastatic proteins are notably stabilized in cancer cells to promote future development, and tumors frequently repeat programs to gain invasive and dispersion capabilities. It is uncertain if unsuccessful protein degradation has an impact on cancer's metastatic programs. It is still mostly unclear if CMA can exhibit degradative selectivity towards metastasis regulators, such as EMT-associated proteins, influencing the cancer cells' capacity to spread and develop further. We used ovarian carcinoma-derived ES2 cells, which exhibit strong genetic similarity to ovarian cancers with mesenchymal characteristics, in a multiplexed quantitative mass spectrometry (MS)-based proteomics technique to experimentally investigate and uncover CMA-targetable proteins causing EMT.