Epigenetic Reactivation of CNS Endothelial Developmental Programs Triggers Adult Brain Angiogenesis, Promotes Post-Stroke Revascularization and Neuroregeneration [RNAseq-Stroke]

We identified the role of brain endothelial cell Hdac2 and Ezh2 mediated epigenetic mechanism in maintaining blood brain barrier in adult mice. Also, we we studied the impact of reactivation of brain endothelial cell Wnt/β-catenin signaling in adult mice. .We evaluated derepression of Hdac2 mediated epigenetic mechanism in imice model of schemic stroke. Ultra-low mRNA sequencing of FACS sorted CD31+ve/CD41-ve/CD45-ve adult mice brain cortical endothelial cells isolated from ipsilateral and contralateral sides of sham, MCAO, MCAO-Hdac2 ECKO and MCAO-Ezh2 ECKO mice. For inducing stroke, middle cerebrai artery occclusion model of stroke was used. For endothelial specific deletion of Hdac2 or Ezh2, Hdac2lox/lox:Cdh5CreERT2 or Ezh2lox/lox:Cdh5CreERT2 mice were treated with Tamoxifen (75mg/Kg body weight), starting day 0 to day4 (total 5 doses) and brains were harvested on 7th day of stroke induction.