Proteomic profiles of Platynereis spp. collected from inside and outside the CO2 vent (Ischia, Italy) and used in a reciprocal transplant experiment in September 2013

Platynereis spp. were collected via snorkelling or scuba from either inside (40°43′53″N, 13°57′47″E) (stations A1-A3) or outside (40°43'33.33"N, 13°57'36.38"E and 40°44′48″N, 13°56′39″E) (stations C1-C3) the carbon dioxide (CO2) vent on the island of Ischia (Italy) and used in a reciprocal transplant experiment. The effect of exposure to high or low partial pressures of CO2 (pCO2) conditions on the proteome of worms from different pCO2 regimes was investigated to understand the effect of exposure to different pCO2 conditions on the cellular physiological response. This experiment was conducted between 04/09/2013 and 16/09/2013. The experiment was staggered during this time so all worms could be processed. After five days exposure to either low or high CO2 conditions worms were snap frozen in liquid nitrogen and shipped to the University of Hong Kong for proteomic analysis which was finalised on 02/08/2015. Proteomic profiles of worms were characterised using a liquid chromatography-mass spectrometry/mass spectrometry triple time of flight (LC-MS/ MS Triple TOF) approach.