DataSheet1_Characterizing CD38 expression in terminally differentiated B cells using variable lymphocyte receptor B tetramers.docx

IntroductionCD38 is an ectoenzyme receptor found on hematopoietic cells and its expression is used in the flow cytometric analysis of sub-populations of circulating B cells among peripheral blood mononuclear cells (PBMC) to aid in diagnosing patients with different antibody production defects (AbD). Monoclonal antibodies derived from the sea lamprey Variable Lymphocyte Receptor B (VLRB) are emerging as an alternative to conventional mammalian antibodies. We hypothesized that VLRB MM3 (V-CD38) which specifically recognizes CD38 in a manner correlating with its enzymatic activity could identify terminally differentiated B cells in human PBMC. Here we investigate the ability of V-CD38 as a tool to diagnose patients with diverse immune abnormalities including AbD.MethodsThe expression of CD38 on CD3-CD19+CD27+ plasmablasts and CD3-CD19+IgMhiCD27- transitional B cells in PBMC was analyzed by flow cytometry using V-CD38 and compared with a commercial conventional antibody to CD38 (C-CD38).ResultsA highly significant correlation (p

CD38是一种存在于造血细胞上的外泌酶受体，其表达被用于外周血单个核细胞（PBMC）中循环B细胞亚群的流式细胞术分析，以辅助诊断具有不同抗体产生缺陷（AbD）的患者。从海鳗可变淋巴细胞受体B（VLRB）中衍生出的单克隆抗体正在成为传统哺乳动物抗体的替代品。我们假设VLRB MM3（V-CD38），该抗体以与CD38的酶活性相关的方式特异性识别CD38，能够识别人类PBMC中的终末分化B细胞。在此，我们探讨V-CD38作为一种工具诊断具有多种免疫异常，包括AbD的患者的能力。方法：通过流式细胞术使用V-CD38分析PBMC中CD3-CD19+CD27+浆细胞和CD3-CD19+IgMhiCD27-过渡B细胞上CD38的表达，并将其与商业常规CD38抗体（C-CD38）进行比较。结果：高度显著的相关性（p