five

Control or KZFP KD hESC-derived organoids RNA-seqs

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NIAID Data Ecosystem2026-03-12 收录
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https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE145406
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Control or KD hESC were differentiated into organoids as decribed in Methods. RNA was extracted from individual organoids at day 20 after commencing the STEMdiff™ Cerebral Organoid Kit protocol. Day 0 was designated as the day of transfer of hESC to a 96-well plate for differentiation into embryoid bodies . hESC were transduced with a control lentivector or a lentivector expressing a sh targeting the KZFPs, selected with puromycin and differentiated into cerebral organoids post-selection.
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2020-09-21
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