Oligonucleotides used for Luminex MOL-PCR.
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1Nomenclature according to [2]. 2Position of SNP in reference to H37Rv. 3SNP in an essential gene (“ess”); synonymous SNP (“syn”). 4Strand orientation of oligonucleotides. 5LPO, left probe oligonucleotide, annealing upstream of SNP of interest and including it; RPO, right probe oligonucleotide, annealing downstream of SNP of interest. 6xTag bead with coupled antiTag-sequence. 7Universal primer sequences (upper case, not underlined), allele-specific tag-sequences (lower case), and sequences hybridizing to the template genomic sequence (underlined). RPO are 5′-phosphorylated.
创建时间:
2012-07-20




