Differentially expressed genes induced with or without UVB stimulation in mice

Skin cancer is the most prevalent cancer in humans, especially in the United States, Australia, and New Zealand. Australia and New Zealand are the two countries with the highest rates of skin cancer in the world, about four times higher than the United States, the United Kingdom and Canada. According to statistics, one American dies of skin cancer every hour. Studies have shown that ultraviolet radiation is the main cause of skin cancer, and ultraviolet rays are mainly divided into UVA, UVB and UVC according to wavelength, UVA and UVB can cause DNA damage, and UVB is the main factor that induces skin cancer. UVB is primarily a direct damage to cellular DNA and generally includes the formation of pyrimidine dimers (CPDs) and 6-4 photoproducts (6-4PPs). UVB can also cause mutations in tumor suppressor genes such as p53, ptch, and ras. These bases the mutation will promote the activation of related signaling pathways, thereby inducing the production of tumors.In this study, we will use gene chip technology to screen out UVB-sensitive genes, and then select the genes of the UVB-sensitive GPCR family from these genes, and further use PCR for verification, so as to identify UVB-sensitive GPCRs, which will provide a basis for further experimental research. The 6-8 week old mice were divided into two groups, a dark-treated (con) and UVB-treated group, with 3 mice in each group. The hair on the back of each mouse was shaved, and after two days, the mice in the UVB-treated group were anesthetized and placed in a UVB irradiation meter to irradiate the back of the mouse with UVB at a dose of 180mj/cm2. Three times a week, irradiation for 2 weeks,The darkened group did not receive UVB exposure.