Circular RNAs in nasopharyngeal carcinoma cell lines with different metastasis potential

Increasing evidence has demonstrated a significant role for circular RNAs (circRNAs) in tumorigenesis. However, their functions in nasopharyngeal carcinoma (NPC) metastasis remain largely unknown. In this study, a model compared high and low metastatic NPC cell lines (S18 vs. S26) was constructed to determine the expression profile of circRNAs using the RNA sequencing analysis. Using Illumina HiSeqTM2000, we performed RNA-sequencing analysis of ribosomal RNA-depleted and RNase-R-treated RNA to acquire different expression profiles of circRNAs in a pair of NPC cell lines with different metastatic potential (the high-metastasis S18 cell and the low-metastasis S26 cell).