Histone mark-ChIP-seq to determine the genome organization of Chamaelirium luteum with large centromeres

To determine the size and sequence composition of the exceptionally large centromeres in the genome of monocentric Chamaelirium luteum, we performed CENH3-ChIPseq using the customized species-specific CENH3 antibody. We mixed the chromatins of C. luteum and Secale cereal (inbred line Lo7) to dilute the highly abundant centromeric Chama satellite repeats in the C. luteum genome before immunoprecipitation. In addition, H3K4me2- and H3K9me2-ChIPseq were performed to verify the large-scale eu- and heterochromatic genome organization.