Transcription profiling of Hsd:ICR(CD-1) mouse intestines to study regional variations in ABC transporter expression along the mouse intestinal tract

8 week-old male Hsd:ICR(CD-1) mice were fed ad libitum a standard chow (Harlan Teklad diet 2018S) and housed in groups of five. Animals were then divided into 3 pools (n=10) and sacrificed. The small intestine was extracted and divided into three sections, where the first 2-3 cm after the stomach comprised the duodenum, the middle third the jejunum, and the section before the ileo-ceco-colic junction comprised the ileum. Colon was not divided into proximal and distal sections and was treated as a single intestinal section.8周龄雄性Hsd:ICR(CD-1)小鼠自由采食标准啮齿类饲料（Harlan Teklad 2018S饲料），并以5只为一组进行饲养。随后将实验动物分为3个组池（每组n=10）并处以安乐死。取出小肠并分为三段：胃后方起始的2~3厘米区段为十二指肠（duodenum），中段三分之一为空肠（jejunum），回盲结肠连接部（ileo-ceco-colic junction）之前的区段为回肠（ileum）。结肠未划分为近端和远端区段，整体作为单一段肠管进行处理。