Single-cell RNA sequencing and spatial seqeuncing of foreign body granuloma

Although macrophage-epithelioid cell (EPC)-giant cell (GC) differentiation is acknowledged in foreign body reaction (FBR), the exact molecular features remain elusive. To discover the molecular profiles of EPC and GC, we analyzed mouse sponge and silk FBRs by integrating single-cell RNA-sequencing (scRNA-seq) and spatial sequencing, which identified seven cell types, including macrophages and fibroblasts.