Gene expression responses to ABA and to osmotic stress in the pyl112458 379101112 mutant

Nearly all of the transcript level responses to ABA in WT seedlings were diminished in the 112458 379101112 mutant. However, only a relatively small percentage of the transcript level responses to osmotic stress was abolished in the 112458 379101112 mutant, and the majority of the responses was maintained, although the levels of up- or down-regulation of many of the transcripts were reduced in the mutant. Wild type and mutant seedlings were grown vertically for 9 days before treatment. These seedlings were mock treated, or treated with 100 µM ABA or 300 mM mannitol for 24 hours on filter papers. The transcriptome were analyzed by deep sequencing, in triplicate, using Illumina HiSeq 2500.