Differential gene expression in MEF SEC24D knockout cells with additional SEC24s knockdown. Differential gene expression in MEF SEC24D knockout cells with additional SEC24s knockdown

We transfected MEF SEC24D KO cells with SEC24B siRNA for a double KD or combined SEC24s siRNA (SEC24a, 24B and 24C) for a quadruple KD. Cells were transfected with siRNA or Scramble siRNA (control) for 2 days then total RNA for each knockdown sample was extracted and purified for RNA-seq to evaluate the change in transcriptome. Overall design: Examination of gene expression in Sec24D knockdown