Supplementary material.docx

Experimental.Plant materials and phytopharmaceutical productChemicals and apparatusPreparation of Standards and samplesInstrumentation and Chromatographic conditionsFiguresOptimisation of chromatographic conditionsS1:Effect of organic solvent type in the mobile phase on separation S2:Effect of initial and final concentration of ACN in the mobile phase on separation S3:Effect of gradient time on separation S4:Effect of column temperature on separation S5:Effect of flow rate on corrected peak area S6:Peak characterization in the crude extracts Indirect-dispersive liquid-liquid microextraction S7: Effect of IDLLME on the baseline, (a) before (b) after IDLLME, Peaks: 1, vitexin 2''-O-rhamnoside, 2, rutin, 3, vitexin, and 4, hyperoside. TablesS1:Optimum chromatographic conditions Determination of analytes in real samples S2:Concentration of analytes in the real samples