Adhesion-derived condensates control component availability to regulate adhesion dynamics

Tensins are key components of integrin adhesions and play a key role in linking the actin cytoskeleton with the ECM through interaction with integrin heterodimers. While tensin 1 (TNS1) localises to both focal and fibrillar adhesions, TNS3 has been mostly associated with fibrillar adhesions. Tensins play a role in maintaining active integrin pools and adhesion reinforcement and facilitate tension-dependent focal adhesion maturation into centrally localized fibrillar adhesions. In addition, TNS1 forms functional and reversible condensates in cells in response to different extracellular stimuli that act as reservoirs of adhesion components. Here we perform proximity biotinylation proteomics (BioID) to identify proximity interactors of TNS1 and TNS3. In parallel, we focus on identifying condensation-dependent proximity interactors of TNS1 in cells with low and high TNS1 expression.