Deciphering NEUROD1's target genes and miRNAs: an integrative analysis in small cell lung cancer

Small cell lung cancer (SCLC) is a particularly challenging tumor to treat, due to its poor prognosis. ASCL1 and NEUROD1 are master regulators that define the molecular subtypes of SCLC, which are characterized by neuroendocrine features. Immunohistochemical studies have demonstrated that these genes can be co-expressed to varying degrees. While the transcriptional functions of ASCL1 have been well-documented, those of NEUROD1, specifically its target genes and miRNAs, remain less explored. The objective of this study was to identify the targets of this gene. Overall design: RNA-seq and miRNA-Seq analyses were conducted on Lu134A following NEUROD1 knockout (KO) to identify gene expression changes relative to untreated (WT) and negative control samples (NC). Furthermore, RNA-Seq analysis was conducted on Lu134A introduced miRNA mimics (negative control (NC) and 139-5p mimic (139)).