Transcriptomic Profiling of Stromal Cell-derived Factor-1 and Exendin-4 Treated Periodontal Ligament Stem Cells Undergoing Osteogenic Differentiation by RNA-Seq

We aimed to evaluate the detailed biological mechanism of SDF-1 and EX-4 alone or synergistic in regulating PDLSCs differentiation by RNA-sequencing (RNA-seq). Overall design: PDLSCs from 5 different individuals were cultured in the osteogenic medium (OM) and treated with SDF-1, Ex-4 or SDF-1+Ex-4 at 21 d, respectively; normal PDLSCs treated with OM was served as control. A total of 20 samples were sequenced to analyze the gene expression at the whole genome level at LC Sceiences (Hangzhou, Zhejiang, China) by RNA-seq.