Single cell sequencing of gastric cancer and adjacent normal tissues

In this study, we used single-cell RNA sequencing (scRNA-seq) to further elucidate the composition of the gastric cancer and adjacent normal tissue microenvironment and found that TNF and ACTG1 may be key regulators of regulatory T cells (Tregs) in gastric cancer. A total of seven cell types were identified, including B cells, T cells, NK cells, mast cells, fibroblasts, endothelial cells, and epithelial cells. T cells were further divided into 8 subgroups. Among them, Tregs showed the greatest difference between GC and adjacent normal tissues. Next, we clarified the function and signaling pathway of Tregs in the GC tumor microenvironment, obtained the key regulatory proteins TNF and ACTG1, and explored their clinical significance and possible effects on Tregs in the GC tumor microenvironment. This scRNA-seq study may reveal the composition of tumor microenvironment and clarify the role of Tregs in the development of gastric cancer, providing new methods for GC treatment. The cancer tissue sample was from a GC patient with untreated, primary, nonmetastatic gastric tumor who underwent GC resection. Adjacent normal gastric tissue was collected more than 5 cm from the carcinoma tissue.