Differential lipid synthesis in the developing seeds of Jatropha and Vernicia: a comparative transcriptomic study

Two Euphorbiaceae oil trees, Vernicia and Jatropha, were chosen to make a comparative transcriptomic study, with a focus on the differential oil accumulation process. Transcriptome sequencing was conducted with seeds at the initial- and fast- stage of oil accumulation from both. Overall design: Seeds of three-year-old Jatropha or Vernicia trees were collected, with the former from a natural farmland in southern Guizhou province of China and the later from northwest Hunan province. Samples collected at two distinct developing stages: the early stage of oil accumulation (S1, Jcu_29DAP, Vfo_90 DAP) and the fast accumulation stage (S2, Jcu_37DAP, Vfo_135DAP). To eliminate the sampling error, total RNAs were extracted independently from three seeds, equally mixed and used for illumina library preparation.