Compounds from the kinase inhibitior library are identified as ABCB1 inhibitors by the high-throughput imaging-based efflux assay.

*Compounds that have been previously reported to interact with ABCB1. References are listed separately (Text S1).
KB-V1 cells treated with the kinase inhibitor library of 193 compounds at 10 µM and 1 µM calcein AM were examined by the IncuCyteTMFLR. The object intensities of each sample were normalized to XR9576 treated cells and expressed as the percentage of inhibition. Compounds that displayed at least 50% inhibition of ABCB1-mediated efflux in all three independent experiments are listed.