Single-cell transcriptomes reveal a complex cellular landscape in the middle ear and differential capacities for acute response to infection gene expression by cell types in the middle ear

Single-cell transcriptomics were used to identify cells in the normal middle ear mucosa, to identify their differentially expressed genes, and to determine cell-type-specific expression of genes related to innate immunity . Three independent samples of pooled murine middle ear mucosa were used to generate 6370 cell transcriptomes, which were analyzed for cell marker, differential and innate immune gene expression.