Single-nucleus RNA-seq of liver tissue of 3 months old mice to investigate crosstalk between ploidy and zonation

Single-nucleus RNA-seq-2 method that allows deep characterization of nuclei isolated from frozen archived tissues. We have used this approach to characterize the transcriptional profile of individual hepatocytes with different levels of ploidy. This method has the potential to explore archived samples, for instance to study the development and progression of disease in complex tissues. To illustrate the potential of this method, we have deeply characterized the cellular heterogeneity driven by spatial distribution and different levels of ploidy in the young adult mouse liver.单细胞核RNA测序2（Single-nucleus RNA-seq-2）技术可实现对冷冻存档组织中分离得到的细胞核进行深度表征。本研究利用该技术对不同倍性水平的单个肝细胞的转录组谱进行了表征。该技术具备探索存档样本的潜力，例如可用于研究复杂组织中疾病的发生与发展进程。为展示该技术的应用潜力，本研究对年轻成年小鼠肝脏中由空间分布及不同倍性水平驱动的细胞异质性进行了深度表征。