Additional file 3: of Gene-specific DNA methylation profiles and LINE-1 hypomethylation are associated with myocardial infarction risk

Supplemental Figures S1, S2, S3, and S4. Figure S1. quantile-quantile plot, EPICOR overall subjects. Figure S2. quantile-quantile plot, EPICOR men. Figure S3. quantile-quantile plot, EPICOR women. Figure S4. locations of ZBTB12 and LINE-1 CpG sites investigated by Sequenom MassARRAY. CpGs (in red) investigated within ZBTB12-DMR, LINE-1, and flanking primers (upper case: complementary to DNA; lower case: T7-promoter sequence and 10mer tag). CpG sites that could not be tested individually due to MassARRAY technology constrains, but had to be tested jointly with neighboring CpGs as a single unit, are underlined: the methylation level is the cumulative value of all the sites within the CpG unit. (ZIP 91 kb)补充图S1、S2、S3及S4。 图S1：EPICOR队列全体受试者的分位数-分位数图（quantile-quantile plot）。 图S2：EPICOR队列男性受试者的分位数-分位数图（quantile-quantile plot）。 图S3：EPICOR队列女性受试者的分位数-分位数图（quantile-quantile plot）。 图S4：本研究通过Sequenom MassARRAY质谱阵列技术检测的ZBTB12与LINE-1的CpG位点定位信息。其中，位于ZBTB12-DMR（差异甲基化区域）、LINE-1及侧翼引物中的CpG位点以红色标注：引物序列的大写部分为DNA互补链，小写部分为T7启动子序列与10mer标签序列。因Sequenom MassARRAY质谱阵列技术限制无法单独检测的CpG位点，需与相邻CpG位点作为单个检测单元联合检测，此类位点以下划线标注：其甲基化水平为该CpG单元内所有位点的累积甲基化值。（ZIP格式压缩包，91 kb）