The CD24+ Cell Subset Promotes Invasion and Metastasis in Human Osteosarcoma

NanoAcquity UPLC(Waters Corp., MA,USA) combined with LTQ Orbitrap XL(Thermo Fisher Scientific Inc., MA, USA) was used to study CD24+ and CD24– subsets of cells isolated from the MNNG/HOS cells using a flow cytometry cell sorter. Collected data were searched in the BioWorksBrowser (version Rev. 3.3.1 SP1, Thermo Fisher Scientific Inc.) with the SEQUEST search engines and analyzed using an APEX Quantitative Proteomics Tool (Version1.0.0, Pathogen Functional Genomics Resource Center；J. Craig Venter Institute)and other bioinformatics software. Through the Database for Annotation, Visualization, and Integrated Discovery (DAVID) and the Kyoto Encyclopedia of Genes and Genomes (KEGG) database platforms, differentially expressed proteins of the CD24+ and CD24- cell subsets were analyzed for KEGG pathways.