Comparative transcriptome analysis between two tomato materials under drought stress

RNA sequencing was performed to investigate the the response mechanism of tomato response to drought stress. C2H2-type zinc finger proteins are classic and extensively studied members of the zinc finger family. C2H2-type zinc finger proteins participate in plant growth, development and stress responses. In this study, 99 C2H2-type zinc finger protein genes were identified and classified into four groups, and many functionally related cis-elements were identified. Differential C2H2-ZFP gene expression and specific responses were analyzed under drought, cold, salt and pathogen stresses based on RNA-Seq data. Thirty-two C2H2 genes were identified in response to multiple stresses. Seven, 3, 5, and 8 genes were specifically expressed under drought, cold, salt and pathogenic stresses, respectively. Five glycometabolism and sphingolipid-related, pathways and the endocytosis pathway were enriched by KEGG analysis. The results of this study represent a foundation for further study of the function of C2H2-type zinc finger proteins and will provide us with genetic resources for stress tolerance breeding. mRNA profiles of different tomato leaf samples were generated by deep sequencing. The sequence reads that passed quality filters were analyzed at the transcript . Please note that the 'Micro-TOM-EX' is the 'Micro-TOM' plants overexpressing a GATA transcription factor gene. CK0*_0h = Micro-TOM_control CK3*_3h = Micro-TOM_drought stress for 3hrs CK6*_6h = Micro-TOM_drought stress for 6hrs EX0*_0h = Micro-TOM-EX_control EX3*_3h = Micro-TOM-EX_drought stress for 3hrs EX6*_6h = Micro-TOM-EX_drought stress for 6hrs