H3K27ac profiling of human tracheobronchial epithelial cells during infection with H1N1, H3N2, and H5N1 influenza virus [ChIP-seq]

Human tracheobronchial epithelial (HTBE) cells are considered to serve as a good correlate of influenza virus infection in the human respiratory tract. ChIP-Seq analysis was used to profile histone acetylation (H3K27ac) in HTBE cells at multiple time points in response to infection with influenza A/California/04/09 (H1N1), A/Wyoming/03/03 (H3N2), and A/Vietnam/1203/04 (H5N1) HALo virus. The Influenza A/Vietnam/1203/04 (H5N1) HALo mutant virus is an attenuated H5N1 virus generated from wild-type Influenza A/Vietnam/1203/04 (H5N1) virus as described in Steel, J., et al. J Virol. 2009 Feb; 83(4):1742-53. Human tracheobronchial epithelial cells were infected with H1N1, H3N2, and H5N1 influenza virus at an MOI of 5. H3N2- and H5N1-infected samples were collected in duplicates at 3, 6, 12, and 18 hours post infection for ChIP-Seq analysis. Sample from H1N1-infected cells were collected at 6, 12, 18, and 24 hours. Uninfected controls (mock infection) were also collected at a single time.