Bulk RNAseq analysis of Ecsit-deficient skeletal progenitors

Whole transcriptome analysis showing differential gene expression in Ecsit sufficient vs deficient skeletal progenitors. Overall design: Skeletal progenitors were obtained from digested hindlimbs of EcsitPrx1;Rosa26mTmG and Ecsitfl/fl;Rosa26mTmG pups at postnatal day 1, and DAPI- live cells were FACS sorted using a FACSAria II SORP cell sorter (Becton Dickinson). Total RNA isolated from Prx1+CD45-Tie2-Ter119- skeletal progenitors was subjected to RNA seq.