Hg-MATE-Db.v1.01142021

<b>Hg-MATE-Db: Hg-</b>cycling<b> M</b>icroorganisms<b> </b>in<b> A</b>quatic<b> </b>and<b> T</b>errestrial<b> E</b>cosystems<b> D</b>ata<b>b</b>ase <br>Microorganisms play a significant role in regulating the form and fate of mercury (Hg) in aquatic and terrestrial ecosystems. Microbes with the <i>hgcAB</i> gene pair can produce a more toxic, and bioaccumulative form of Hg, methylmercury (MeHg). Microbes that possess the <i>mer</i> operon can demethylate and/or reduce Hg species as part of a detoxification mechanism. Improved techniques for capturing <i>hgcAB</i> and <i>mer </i>presence and diversity are necessary for identifying the major microbial players in environmental Hg cycling. The primary goal of the Hg-MATE-Db is to provide an up-to-date collated resource of Hg-cycling genes from pure culture and environmental microbial genomes and meta-omic datasets. The database will be updated regularly.<br><br>The Hg-MATE-Db.v1 contains an<b> </b><i>hgcAB </i>dataset with resources for identifying key microbial producers of the toxin MeHg. Future versions of the Hg-MATE-Db<b> </b>will also include <i>hgcAB</i> sequences from high-throughput sequencing and clone datasets. Future versions will also contain a <i>mer </i>dataset, which will contain resources for identifying genes of the <i>mer-</i>operon that encode for demethylation of organomercurials (<i>merB</i>), reduction of inorganic Hg(II) (<i>merA</i>), as well as operon regulation (<i>merR</i>), and Hg transport across the cell (<i>merTPC</i>).<br>The latest release, version 1 (v1), was compiled on 23 October 2020 and finalized on 14 January 2021, and contains an <i>hgcAB </i>dataset. The catalogue contains 1053 unique HgcA/B amino acid sequences. <br><br> Please cite us when using the Hg-MATE-Db in your work. Gionfriddo, C., Capo, E., Peterson, B., Lin, H., Jones, D., Bravo, AG., Bertilsson, S., Moreau, J., McMahon, K., Elias, D., and Gilmour, C. (2021). Hg-MATE-Db.v1.01142021. doi:10.25573/serc.13105370

**Hg-MATE-Db：水生与陆地生态系统汞循环微生物数据库**

微生物在调控水生与陆地生态系统中汞（mercury, Hg）的形态与归趋方面发挥着关键作用。携带hgcAB基因对的微生物可合成毒性更强、生物累积性更高的汞形态——甲基汞（methylmercury, MeHg）。而携带mer操纵子（mer operon）的微生物则可通过脱甲基化或还原汞物种的方式实现解毒。要精准识别环境汞循环中的核心微生物类群，亟需优化用于检测hgcAB与mer基因的存在及多样性的技术手段。Hg-MATE-Db的核心目标是提供一套最新的整合资源库，涵盖纯培养微生物及环境微生物基因组、宏组学（meta-omic）数据中的汞循环相关基因，且该数据库将定期更新。

Hg-MATE-Db v1版本包含hgcAB数据集，可用于识别甲基汞这一毒素的核心微生物生产者。后续版本的Hg-MATE-Db还将纳入高通量测序与克隆文库数据集中的hgcAB序列；此外还将增设mer数据集，该数据集可用于识别mer操纵子的各类功能基因：包括编码有机汞脱甲基化的merB基因、无机汞(II)还原的merA基因、操纵子调控的merR基因，以及跨细胞汞转运的merTPC基因。

该数据库最新正式版本为v1，于2020年10月23日完成汇编、2021年1月14日最终定稿，仅收录hgcAB数据集。该数据集共包含1053条独特的HgcA/B氨基酸序列。

若您在研究中使用Hg-MATE-Db，请引用如下文献：Gionfriddo, C., Capo, E., Peterson, B., Lin, H., Jones, D., Bravo, AG., Bertilsson, S., Moreau, J., McMahon, K., Elias, D., and Gilmour, C. (2021). Hg-MATE-Db.v1.01142021. doi:10.25573/serc.13105370